Frequently Asked TechTALK Questions
How many mRNA isolations can be run with the MPG™ Direct mRNA Purification Kits?
Can the amount of mRNA isolated using the mRNA Isolation Kits be increased by reusing the particles?
I want to use an immobilized capture probe to isolate a specific DNA species. Which product is best suited for this purpose?
How much nucleic acid can be covalently coupled to MPG™ LCA using the Solid Phase Oligo COUPLE-IT Kit?
How do I optimize my PCR conditions when using the Taq-FORCE DNA Amplification System?
I want to use the SOLID
Solid Phase cDNA Synthesis Kit to construct immobilized cDNA libraries. Which RNA isolation kit can I use that is compatible with this solid phase cDNA synthesis kit?
How much mRNA can I expect to isolate from tissue or cells? What is the proportion of mRNA to total RNA?
Why does isolated RNA degrade so easily and how can I prevent this?
How can I efficiently produce a cDNA library without vector background?
By what methodology does PureBiotech LLC's DNA-Pure PCR Clean Up Kit purify PCR products?
I have been using PureBiotech LLC's Solidscript Solid Phase cDNA Synthesis Kit to study gene expression. I now want to isolate specific rare messenger RNAs using complementary probes. Can I still use this kit?
I am synthesizing a 60' mer 5' amine-modified oligonucleotide to be used to covalently couple to magnetic particles using the Solid Phase Oligo Couple-IT Kit. Since only approximately 60% of the oligos will be full length, do I need to purify the oligo before coupling?
There are many different ratios and pH of phenol:chloroform some with isoamyl alcohol. How do I choose the correct formulations?
During DNA isolation I have a hard time recovering the aqueous phase without contamination from the interface of the phenol/chloroform extraction step. How can I avoid this contamination and still recover the entire DNA from the aqueous phase?
I need to generate a cDNA library from only a few milligrams of an aortic tissue biopsy. Is this possible using your MPG™ mRNA Purification Kits and can I scale your protocols down linearly?
I want to purify protein using your Spin-Pure Columns. How do I decide which type to use?
I have a peptide, which binds to a factor in serum. Can I conjugate this peptide to MPG™ Long Chain Alkylamine to affinity purify the factor?
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