Researcher: I want to purify protein using your Spin-Pure™ Columns. How do I decide which type to use?

TechTALKer:PureBiotech LLC Spin-Pure™ Columns provide fast and simple non-quantitative recovery when used to purify proteins. The following table gives the observed recoveries for various sized proteins using 25 µl of solutions at concentrations of 2.5-3.0 mg/ml. As you can see, the G-50 Column would be the choice for proteins in the range of 150 kDa, while the G-25 Column performs better for proteins in the 66 kDa range. Spin-Pure™ Columns are recommended for proteins >42 kDa. For all proteins, removal of buffer salts is >99% as determined by dye separation or removal of radiolabeled low molecular weight solutes. An extra 10% protein recovery can be obtained by loading an additional 25 µl of water and re-centrifuging, but this will reduce the removal of salts to ~98%.

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• Spin-Pure™ Columns