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Researcher: How much mRNA can I expect to isolate from tissue or cells? What is the proportion of mRNA to total RNA? |
TechTALKer:The amount of mRNA and the proportion of mRNA to the total RNA present in cells and tissue varies with the cell type and tissue. The following table adapted from Alberts et al, Molecular Biology of the Cell (Third Edition, Garland Publishing, NY) provides a summary of the RNA content in various cells and mouse tissues.
| Source | Total RNA (µg) | mRNA (µg) | % mRNA |
|---|---|---|---|
| Cell Culture (107 cells) | |||
| NIH 3T3 | 150 | 3 | 2.0 |
| HeLa | 100-300 | 2-6 | 2.0 |
| CHO | 200-400 | 3-6 | 1.5 |
| Mouse Tissue (100 mg) | |||
| brain | 200 | 7 | 3.5 |
| heart | 90 | 6 | 6.7 |
| intestine | 300 | 3 | 1.0 |
| kidney | 300 | 9 | 3.0 |
| liver | 700 | 14 | 2.0 |
| muscle | 125 | 1-2 | 0.8-1.6 |
| lung | 130 | 10 | 7.7 |
| spleen | 600 | 7 | 1.2 |
PureBiotech LLC offers a variety of kits which allow you to isolate total RNA, mRNA directly from cells or tissue, or mRNA from total RNA. The end result of each kit is pure, intact total RNA or mRNA with purities exceeding 1.80 and 1.90 respectively. Each kit is scaleable, up or down, to offer you the flexibility to isolate the amount of RNA needed for your application. This provides a cost saving to you by reducing the amount of reagents used for small isolations.
Related Product Pages
- MPG® Direct mRNA Purification Kit
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