Controlled Pore Glass Products

About Controlled Pore Glass

Controlled Pore Glass (CPG)1 is produced from a borosilicate base material which is heated to separate the borates and the silicates. The borates are leached out from the material, leaving the silica glass with uniform, controlled pores. Over time, manufacturing innovations and improvements, notably including those of Wolfgang Haller, led to the widespread adoption of the support in application areas such as chromatography, solid phase extraction and solid phase synthesis. Even in today's world of performance polymers and nanomaterials, CPG continues to be a benchmark. CPG offers many advantages over other solid phases. These include physical, chemical, and thermal stability, high strength, high surface area and uniform pore size distribution. Furthermore, through the use of silane chemistry, a virtually unlimited repertoire of surface properties and chemical functionality can be conferred on the support. The pore size of CPG ranges from 75 through 3000 angstroms, and the particle size ranges from 1 through more than 200 microns. The bioprocessing industry typically employs supports having pores greater than 500 angstroms and particle sizes in excess of 75 microns since these particles support the high flow rates required. As the starting material for Magnetic Porous Glass (MPG™) PureBiotech stocks certain novel forms of CPG, namely particles of less than 75 microns diameter in pore sizes of 500 and 1000 angstroms. These smaller particles are more appropriate for analytical work and batch applications.


Custom Ligand Immobilization

We will perform ligand immobilization using the chemistries outlined in Table 1 on CPG or MPG™ supports that you specify. We also offer the supports indicated in the table should you wish to perform the coupling chemistry yourself.  Most are provided on a custom basis although some may be in stock.  Please
inquire for current inventory.  Prices include a $1000 minimum and depend upon the specifics of the project. Click here to discuss your custom immobilization project with our specialists.

Table 1.   Immobilization Chemistries
Coupling of Ligands via Functional Group of Ligand to be Coupled Reagent for Particle Activation MPG™/CPG Support Type
Amide Bond -NH2 EDAC, NHS Carboxyl-CPG
Alkylamine Bond -NH2 Sodium Periodate Glyceryl-CPG
Alkylamine Bond -NH2 Glutaraldehyde Aminopropyl or Long Chain Alkylamine-CPG
Amide Bond -Anhydride None Aminopropyl or Long Chain Alkylamine-CPG
Thiourea Bond -NCS None Aminopropyl or Long Chain Alkylamine-CPG
Disulfide Bond -SH Pyridyl Disulfides Thiopropyl-CPG
Diazo Bond -Phenol or ØNH2 Nitrous Acid Aminoaryl-CPG
Hydrazone Bond -CHO None Hydrazide-CPG
Conjugation Streptavidin-Antibody, Avidin-Antibody, Streptavidin-Oligonucleotide, Avidin-Oligonucleotide None Biotin-CPG

1As used throughout this website CPG refers to the generic acronym for controlled pore glass. CPG™ is a registered trademark of EMD Millipore Corporation which refers to their specific manufactured brand of controlled pore glass.


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