The MPG™ mRNA Purification Kit is designed specifically for the isolation of pure, intact mRNA from purified total RNA utilizing magnetic separation technology. The isolation takes 15 minutes or less. The kit contains all the reagents necessary to isolate a typical range of 30-65µg of high quality mRNA in a single round of isolation. Increased yields are obtained from pooling multiple rounds of mRNA isolated from the same sample, using the same particles.
The key component of the kit MPG™ Streptavidin Complex, provides greater surface area than other magnetic particles, resulting in greater oligo (dT) binding and therfore generating greater mRNA capture. The mRNA captured is released in water with purities >1.8 as measured by A260/A280. The purified mRNA is compatible with applications including cDNA library construction; dot blot hybridization; Northern blotting; RNA binding protein isolation; S1 nuclease analysis; in vitro translation; subtractive hybridizations; cloning; sequencing; and RT-PCR.
© 2004-2019 PureBiotech LLC All rights reserved.