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MPG™ Direct mRNA Purification Kit
Back to MBPDBack to RNA Purification

  • Isolate pure, intact mRNA directly from cells or tissue in 15 minutes
  • Magnetic separations allow purification in a single tube
  • No need for messy oligo(dT) columns
  • Contains enough reagents to isolate a typical range of 30-65 µg of mRNA
  • Increased yields can be obtained from pooling multiple rounds of mRNA isolated from the same tissue, using the same particles

MPG™ Direct mRNA Purification Kit - non toxic & Phenol Free
For isolation of mRNA directly from cells and animal or plant tissue.

MPG™ Guanidine Direct mRNA Purification Kit - Phenol Free
For isolation of mRNA from cells or animal tissue containing high levels of RNase.

The MPG™ Direct mRNA Purification Kits are designed specifically for the isolation of pure, intact mRNA directly from cells and animal or plant tissue. The isolation takes 15 minutes or less and eliminates the need for total RNA isolations, oligo(dT) columns, phenol-chloroform extractions and long overnight centrifugations through CsCl gradients. The kits contain all of the reagents necessary to isolate and purify high quality mRNA up to 1.0 gram of tissue or 1 x 108 cells. A typical range of 30-65 µg of mRNA can be purified per kit. Increased yields are obtained from pooling multiple rounds of mRNA isolated from the same tissue, using the same particles.

The key component of the kits, MPG™ Streptavidin Complex, provides greater surface area than other magnetic particles, resulting in greater oligo(dT) binding and, therefore, generating greater mRNA capture. Typical yields of 3-7 µg/mg of solid phase are achieved from as little as 0.05 grams of tissue (mouse liver). The messenger RNA is captured directly from tissue or cell homogenates on oligo(dT)25 - bound MPG™ Streptavidin and released in water with purities >1.8 as measured by A260/A280.

The mRNA purified by these kits is ready for direct use in: cDNA library construction; Northern blotting; in vitro translation; S1 nuclease analysis; sequencing; dot blot hybridization; RNA binding protein isolation; subtractive hybridization; cloning; and RT-PCR.

SAMPLE
mouse
tissue
Yield mRNA
µg/mg MPG™
Streptavidin
Complex
Purity mRNA
A260/A280
Liver 4.01 1.9
Brain 3.4 1.8
Kidney 3.4 1.8
Lung 3.4 1.8

mRNA yield is dependent on the origin of the tissue.

mRNA was isolated from fresh mouse tissues homogenized in Tissue Extraction/Hybridization Buffer (0.05 g/ml). 1 ml homogenates were incubated with 1 mg MPG™ Streptavidin-oligo(dT)25 Complex. (following protocol 5.2)

Related Sections:

Catalog # Description Unit Price
MDRK1010 MPG™ Direct mRNA Purification Kit 1 Kit $270.00
MDRK1011 MPG™ Direct mRNA Purification Kit including 3-in-1 MPS™ (Magnetic Particle Separator) 1 Kit $450.00
MGRK1010 MPG™ Guanidine Direct mRNA Purification Kit 1 Kit $280.00
MGRK1011 MPG™ Guanidine Direct mRNA Purification Kit including 3-in-1 MPS™ (Magnetic Particle Separator) 1 Kit $455.00

 

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