Taq-FORCE Amplification System includes unmodified Taq-FORCE DNA Polymerase, dNTP Mix and our special MIGHTY Buffer. The Taq-FORCE Amplification System has been demonstrated to outperform standard Taq DNA polymerase systems in PCR reactions. The MIGHTY Buffer, our special 10X Reaction Buffer, is complete with MgCl2 (to give a final concentration of 1.5 mM), and unique enhancers allowing for efficient amplification of difficult templates.
Taq-FORCE DNA polymerase is the native enzyme isolated from Thermus aquaticus YT-1 (Kaledin, A.S. et al (1980) Biokhimiia 45:644). Taq-FORCE DNA Polymerase is supplied at either 250 U/vial (5 U/µl) or 500 U/vial (15 U/µl). Taq-FORCE Amplification System 250 comes with enough reagents to carry out 100 50-µl PCR reactions (2.5 U/reaction). Taq-FORCE Amplification System is optimal for use with our SOLIDscript Solid Phase cDNA Synthesis Kit for RT-PCR.
Comparison of Taq-FORCE Amplification System to a leading competitor's system. DNA template, containing a region of the mouse p53 gene, was added to PCR reactions in amounts ranging from 10 to 100,000 copies. 50-µl PCR reactions were performed using 2.5 units of Taq and the buffer supplied with each system. PCR products were resolved on a 1.2% agarose gel and visualized by ethidium bromide-staining.
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