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Product No. Product Description Unit Price
MB0250 MIGHTY™ Buffer 2 x 1 ml $ 30.00
  APPLICATIONS:
  • Efficient amplification of immobilized DNA
  • Reactions in glass capillaries
  • Reactions in situ on glass slides
  • Templates with secondary structure and other difficult templates

MIGHTY™ Buffer is a proprietary 10X formulation of enhancers and additives that promote effective PCR amplification in the presence of silica surfaces. MIGHTY™ Buffer reduces non-specific interactions of all reaction components, increases enzyme and template thermostability, and reduces non-specific priming. This results in increase of sensitivity, yield, and specificity. MIGHTY™ Buffer is complete with MgCl2 to a final concentration of 1.5 mM.

MLB0250 MIGHTY-L™ Buffer 1 ml $ 45.00
  APPLICATION:
  • Efficient amplification of long templates

Our special 10X Reaction Buffer enhanced and optimized to allow for efficient amplification of long templates. This non-tris buffer increases enzyme half-life and provides optimal pH control to provide higher DNA yield. MIGHTY-L™ Buffer can simply be substituted for the incubation buffer currently used. Mg2+ is added separately to the reaction.

NSA0250 No-Smear Additive 3 X 1.2 ml $ 30.00
  APPLICATION:
  • Specificity enhancer

No-Smear Additive is a proprietary compound that eliminates background smears and spurious bands on difficult templates such as GC-rich and repetitive sequences. This additive acts by isostabilizing the melting temperatures of GC and AT base pairs. It can be used to improve specificity in almost any reaction. No-Smear Additive is supplied as a 5X concentrate and should be used at a final empirically determined concentration of 0.5 to 2X.

NHB0250 10X NH4 Reaction Buffer 3 X 1.2 ml $ 16.00
  This buffer provides superior yield with lower background as compared to KCl-based buffers. Yields may be up to 2 times greater with no loss of specificity or fidelity. 10X NH4 Buffer consists of 160 mM (NH4)2SO4, 670 mM Tris-Cl (pH 8.8 at 25°C), 0.1% Tween-20.
KCB0250 10X KCl Reaction Buffer 3 X 1.2 ml $ 16.00
  10X KCl Buffer is 500 mM KCl, 100mM Tris-HCl (pH 8.0 at 25°C), 1% Triton X-100. Suitable for use with Release-IT™.
MGB0250 50mM MgCl2 Solution 3 X 1.2 ml $ 16.00
  This 50mM MgCl2 Solution is supplied in nuclease free water.

 

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