Figure 2: Differential expression analysis by solid phase RT-PCR. Immobilized cDNA libraries were constructed from the liver, lung, heart, and kidney of a single mouse using the SOLIDscript Solid Phase cDNA Synthesis kit. Differential gene expression levels of ß-actin, IL-1ß and p53 in the cDNA libraries were analyzed by PCR using the Taq-FORCE Amplification System. PCR products were resolved on a 1.2% agarose gel (1X TAE) and visualized by EtBr staining. Figure 1: Comparrison of solid phase- versus solution-based RT-PCR. Figure 3: Specificity of Solid Phase RT-PCR.
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