Figure 1: Comparrison of solid phase- versus solution-based RT-PCR. cDNA libraries from a single mouse liver were constructed on a magnetic solid support using SOLIDscript (Lane 1) or in solution from isolated mRNA (Lane 2). Equal amounts of cDNA were analyzed for IL-1ß expression by PCR. PCR products were resolved on a 1.2% agarose gel (1X TAE) and visualized by EtBr staining. Figure 2: Differential expression analysis by solid phase RT-PCR. Figure 3: Specificity of Solid Phase RT-PCR.
© 2004-2019 PureBiotech LLC All rights reserved. |