SPIN-Pure Columns are specifically designed for the fast and efficient separation of large molecules from small molecules. These columns are economical, easy to use, fast and non-toxic. SPIN-Pure Columns provide a superior alternative to common techniques such as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.
Processing of samples is quick and easy. The SPIN-Pure Columns are briefly spun to remove the interstitial fluid. The sample is then added and centrifuged through the column. SPIN-Pure Columns remove contaminant molecules based on size exclusion chromatography. Molecules larger than the matrix pore size are excluded from the matrix and pass directly into the collection tube. Smaller molecules are retained within the column matrix. Greater than 98% of the contaminants are retained in the resin. After processing, your sample is free of contaminants and is recovered undiluted. Adsorption, washing, or special elution steps are not required and the fast 5 minute procedure gives you up to 90% sample recovery.
SPIN-Pure Columns are microcentrifuge compatible and can process sample volumes of 20 ΅l to 50 ΅l. The columns are supplied ready-to-use, pre-packed and pre-hydrated to save you time. They are packed with Sephadex G-25 or Sephadex G-50 size exclusion media and hydrated in your choice of DEPC-water for safe RNA purifications or with TE Buffer. SPIN-Pure Columns are also available packed with dry Sephadex G-25 or G-50 and can be hydrated with reagent-grade water or any suitable buffer prior to use. Each unit comes complete with wash and sample collection tubes.
The SPIN-Pure G-25 Columns will provide excellent recovery of DNA fragments >10 bases. SPIN-Pure G-25 Columns have been designed specifically for the following uses:
SPIN-Pure G-50 Columns provide excellent recovery of DNA fragments >20 bases. SPIN-Pure G-50 Columns have been designed specifically for the following uses:
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