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- Rapid, efficient and easy-to-use
- No sample dilution
- Sample sizes ranging from 20-50 ΅l
- Non-toxic
- Pre-packaged in a variety of pre-hydrated and dry formats
- Excellent for desalting oligonucleotide
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SPIN-Pure Columns are specifically designed for the fast and efficient separation of large molecules from small molecules. These columns are economical, easy to use, fast and non-toxic. SPIN-Pure Columns provide a superior alternative to common techniques such as phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.
Processing of samples is quick and easy. The SPIN-Pure Columns are briefly spun to remove the interstitial fluid. The sample is then added and centrifuged through the column. SPIN-Pure Columns remove contaminant molecules based on size exclusion chromatography. Molecules larger than the matrix pore size are excluded from the matrix and pass directly into the collection tube. Smaller molecules are retained within the column matrix. Greater than 98% of the contaminants are retained in the resin. After processing, your sample is free of contaminants and is recovered undiluted. Adsorption, washing, or special elution steps are not required and the fast 5 minute procedure gives you up to 90% sample recovery.
SPIN-Pure Columns are microcentrifuge compatible and can process sample volumes of 20 ΅l to 50 ΅l. The columns are supplied ready-to-use, pre-packed and pre-hydrated to save you time. They are packed with Sephadex G-25 or Sephadex G-50 size exclusion media and hydrated in your choice of DEPC-water for safe RNA purifications or with TE Buffer. SPIN-Pure Columns are also available packed with dry Sephadex G-25 or G-50 and can be hydrated with reagent-grade water or any suitable buffer prior to use. Each unit comes complete with wash and sample collection tubes.
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Column Specifications |
| G-25 |
G-50 |
| Recovery of DNA Fragment: |
>10 bases |
>20 bases |
The SPIN-Pure G-25 Columns will provide excellent recovery of DNA fragments >10 bases. SPIN-Pure G-25 Columns have been designed specifically for the following uses:
- Desalting
- Buffer Exchange
- Removal of low molecular weight
precursors and contaminants
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| Table 1. Experimental Results G-25: |
| Size of DNA |
% Recovery |
| NTP |
<8 |
| 11-mer |
64 |
| 15-mer |
71 |
| 20-mer |
71 |
| 24-mer |
67 |
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SPIN-Pure G-50 Columns provide excellent recovery of DNA fragments >20 bases. SPIN-Pure G-50 Columns have been designed specifically for the following uses:
- Removal of unincorporated precursors in random-priming, nick-translation and end labeling reactions
- Removal of dye deoxyterminators in manual
or automated sequencing reactions
- Purification of protein conjugates in
iodination, cross-linking, biotin and fluorescence labeling, and
haptenation
- Desalting/purification/buffer exchange of
proteins prior to electrophoresis
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| Table 2. Experimental Results G-50: |
| Size of DNA |
% Recovery |
| NTP |
<0.01 |
| 11-mer |
<2 |
| 15-mer |
39 |
| 20-mer |
72 |
| 24-mer |
72 |
| 28-mer |
81 |
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Spin-Pure Columns provide fast and simple non quantitative recovery when used to purify proteins. Table 3 gives the observed recoveries for various sized proteins using 25 ΅l of solutions at concentrations of 2.5-3.0 mg/ml. Spin-Pure Columns are recommended for proteins >42 kd. For all proteins, removal of buffer salts is >99%. An extra 10% protein recovery can be obtained by loading an additional 25 ΅l of water and re-centrifuging, but this will reduce the removal of salts to ~98%.
| Table 3: Recovery (%) of Protein from Spin-Pure Columns. |
| Protein |
MW (kDa) |
% Recovery |
| G-25 |
G-50 |
| IgG |
150 |
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55-87 |
| BSA |
66 |
62-70 |
14-29 |
| Protein A |
42 |
13-27 |
13-18 |
| Product No. |
Format |
Column Packing |
Quantity |
Price |
| SCD50-50 |
Dry |
Sephadex G-50 |
50 |
$138.00 |
| SCT50-50 |
TE Buffer |
Sephadex G-50 |
50 |
$138.00 |
| SCW50-50 |
DEPC-water |
Sephadex G-50 |
50 |
$155.00 |
| SCD25-50 |
Dry |
Sephadex G-25 |
50 |
$138.00 |
| SCT25-50 |
TE Buffer |
Sephadex G-25 |
50 |
$138.00 |
| SCW25-50 |
DEPC-water |
Sephadex G-25 |
50 |
$155.00 |
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Packs Include:
- 50 pre-packed spin columns
- 50- 2.0 ml wash tubes
- 50- 1.5 ml sample collection tubes
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