• Rapid, gentle & complete immobilization
• High yields of concentrated target protein
• Broad, strong and IgG-specific binding spectrum
• Binding specificity complements MPG™ Protein A
• Easily automated

MPG^™ Protein G is particulate magnetic porous glass with Protein G coupled through stable imide bonds to the surface, allowing quick, reliable, and reproducible magnetic separation of IgG (or target antigen) from solution. The IgG binding properties of MPG^™ Protein G are identical to Gammabind G Type II¹, a truncated form of Protein G containing 2 IgG binding "B domains" and the highly charged "C region". Because it lacks the albumin and Fab binding sites² present in the amino terminus and the hydrophobic membrane anchor region in the carboxyl terminus of the full length protein³, MPG^™ Protein G is highly specific for the Fc region of IgG and has very low non-specific binding properties. The binding capacity of 1 mg of particles is between 50-75 µg.

MPG^™ Protein G is particularly well suited to applications where the strongest possible IgG binding is. It is also well suited to capture and washing at relatively low pH (4-6), which adds versatility to the design of affinity purification protocols.

Use of magnetic particles eliminates the need for centrifugation, spin baskets, and columns. Magnetic particles are also easier to automate and provide greater flexibility in assay configuration. This format reduces assay/purification time by promoting rapid and complete washing involving few steps and a single tube. Magnetic porous glass technology also allows the purified target protein to be recovered in a more concentrated form compared to these formats or compared to immobilized Protein G microplates. Microplates require the use of a large portion of the well to achieve adequate binding capacity and this necessitates large elution volume to recover antibody/antigen. In contrast, MPG^™ Protein G possesses a large surface area in a relatively small volume. This makes MPG^™ Protein G the clear choice for Microplate based applications.

APPLICATIONS:

• Small scale affinity purification
• IgG, Fab, Fc purification
• Immunoprecipitation reactions
• Single or automated immunoassays & bioassays
• Separation of unbound labeled tracers
• Oriented IgG conjugation
• Protein sequencing

RELATED SECTIONS:

• Magnetic Technology
• Antibody Binding Guide, Protein A & Protein G Selection Guide
• Magnetic Separators

¹ McGuire, J. (1989) ABL 7:28.
² Erntell, M. et al (1988) Molecular Immunology 25:121.
² Fahnestock, S.R. etal (1986) J. Bacteriaol 167:870.