Index: Volume 5 Number 1
Next Article: Now Faster And Easier mRNA Isolation

• Multiple Samples at One Time

• High Yield & Purity

• No Ethanol Precipitations or Organic Solvents

• Easy Sample Tracking

• Low Cost

PureBiotech LLC microplate PCR^* kits combine the ease and convenience of advanced separation technology chemistry with the speed of multi-plexed 96 or 384-well microplate centrifugation format. These kits routinely provide >90% yield, >99.9% purity, <0.01% well-to-well contamination and high-throughput (> 1,000 purified PCR products per day).

Figure 1: PCR products purified with our kit. Human cDNA inserts were amplified by PCR, purified with our 96-well PCR purification Kit and 10% of each sample (lanes 2-13),together with molecular weight markers (lane 1), were fractionated on 0.8% agarose, stained with ethidium bromide and photographed. Fluorescent image is represented in pseudo color scale.

The kit utilizes a Binding Buffer, when combined with PCR products allows double-stranded DNA to selectively bind to the Super Filter membrane plate. The Binding Buffer provides a pale blue appearance that facilitates sample mixing and tracking, and assists in monitoring subsequent wash steps. The Wash Buffer selectively rinses away contaminants such as primers, nucleotides, salts, proteins and other impurities. The bound PCR product is eluted yielding highly purified DNA. The DNA purified with this kit is suitable for direct use in DNA Chips, Gene Arrays, DNA Microarrays, High Density Filters, Ink-Jetting Delivery, Gene Mapping, DNA Sequencing, Deletion Studies and other applications in genomics.

^*The PCR process is covered by a series of U.S. and foreign patents issued to Hoffman-LaRoche, Inc.

Next Article: Now Faster And Easier mRNA Isolation