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- Rapid and gentle separations
- Eliminates tedious centrifugation and
chromatography steps
- Cost effective
- Simplifies protocols
- Easily scaled and automated
MPG™ secondary antibody particles are Magnetic Porous Glass (MPG™) particles to which affinity purified secondary antibodies have been covalently attached. Depending on the secondary antibody, the nominal binding capacity of 1 mg of particles is between 40-70 µg of immunoglobulin. MPG™ particles bind immunoglobulins from a variety of species including; mouse, human, rabbit, rat, goat, and sheep primary antibodies (Figure 1). The selection of a secondary antibody particle is dependent on the species and class of the target antibody.
MPG™ secondary antibody particles eliminate centrifugation, allow easier scale-up and automation, and give flexibility in assay configuration. These particles can be used as solid supports in single or automated enzyme and radio immunoassays. They are highly recommended for separating unbound labeled tracer. These particles are excellent in small-scale immunoaffinity purifications or immunoprecipitation reactions for gel or other physical analyses. Use of magnetic particles not only reduces assay time, but as a result increases performance. These particles should be used in conjunction with a magnetic particle separator to achieve purification in minutes.
APPLICATIONS
- Immunoassays - RIA, EIA, ELISA
- Separation of Unbound Labeled Tracers
- Small Scale Immunoaffinity Purification
- Immunoprecipitation Reactions
Figure 1. Typical Attachment of an Antibody to MPG™ Secondary Antibody Particles.
Description | Catalog # | Unit | Price |
MPG™ Goat-anti Mouse IgG (H&L)
|
G-AB11020
| 2 ml
| $235.00
|
G-AB11100 | 10 ml | $750.00 |
MPG™ Goat-anti Mouse IgG F(c)
| G-AB12020
| 2 ml
| Inquire*
|
G-AB12100 | 10 ml | Inquire* |
MPG™ Goat-anti Mouse IgM mu
| G-AB13020
| 2 ml
| Inquire*
|
G-AB13100 | 10 ml | Inquire* |
MPG™ Goat-anti Human IgG F(c)
| G-AB21020
| 2 ml
| Inquire*
|
G-AB21100 | 10 ml | Inquire* |
MPG™ Goat-anti Rabbit IgG F(c)
| G-AB31020
| 2 ml
| Inquire*
|
G-AB31100 | 10 ml | Inquire* |
MPG™ Goat-anti Rat IgG (H&L)
| G-AB41020
| 2 ml
| Inquire*
|
G-AB41100 | 10 ml | Inquire* |
MPG™ Donkey-anti Rabbit IgG (H&L)
| D–AB32020
| 2 ml
| Inquire*
|
G-AB32100 | 10 ml | Inquire* |
MPG™ Donkey-anti Goat IgG (H&L)
| D-AB51020
| 2 ml
| Inquire*
|
G-AB51100 | 10 ml | Inquire* |
MPG™ Donkey-anti Sheep IgG (H&L)
| D-AB61020
| 2 ml
| Inquire*
|
G-AB61100 | 10 ml | Inquire* |
MPG™ Rabbit-anti Human IgG (H&L)
| R-AB22020
| 2 ml
| Inquire*
|
G-AB22100 | 10 ml | Inquire* |
Components:
Packed as 1% suspension in PBS with biocide and BSA. |
*Please Inquire for more Information.
RELATED PRODUCTS
Catalog # | Description | Unit | Price |
MPS0096 |
96-Well MPS™ (for 96-well microplates) |
1 |
$300.00 |
MPS0301
| 3-in-1 MPS (holds 8 x 1.5 ml tubes or 1 x 15ml & 1 x 1.5 ml tube or 1 x 50 ml & 1 x 1.5 ml tube)
| 1 | $250.00
|
MPS0001
| Mini Separator (holds 1 x 1.5 ml tube)
| 1
| $40.00
|
- UHMW polyethylene magnetic particle separator containing neodymium-iron-boron magnets
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