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MPG™ Magnetic Secondary Antibody Patricles
Back to MBPDBack to Affinity Capture of Molecules

  • Rapid and gentle separations
  • Eliminates tedious centrifugation and chromatography steps
  • Cost effective
  • Simplifies protocols
  • Easily scaled and automated

MPG secondary antibody particles are Magnetic Porous Glass (MPG) particles to which affinity purified secondary antibodies have been covalently attached. Depending on the secondary antibody, the nominal binding capacity of 1 mg of particles is between 40-70 µg of immunoglobulin. MPG particles bind immunoglobulins from a variety of species including; mouse, human, rabbit, rat, goat, and sheep primary antibodies (Figure 1). The selection of a secondary antibody particle is dependent on the species and class of the target antibody.

MPG secondary antibody particles eliminate centrifugation, allow easier scale-up and automation, and give flexibility in assay configuration. These particles can be used as solid supports in single or automated enzyme and radio immunoassays. They are highly recommended for separating unbound labeled tracer. These particles are excellent in small-scale immunoaffinity purifications or immunoprecipitation reactions for gel or other physical analyses. Use of magnetic particles not only reduces assay time, but as a result increases performance. These particles should be used in conjunction with a magnetic particle separator to achieve purification in minutes.

APPLICATIONS

  • Immunoassays - RIA, EIA, ELISA
  • Separation of Unbound Labeled Tracers
  • Small Scale Immunoaffinity Purification
  • Immunoprecipitation Reactions

Figure 1. Typical Attachment of an Antibody to MPG Secondary Antibody Particles.

DescriptionCatalog #UnitPrice
MPG Goat-anti Mouse IgG (H&L) G-AB11020 2 ml $235.00
G-AB1110010 ml$750.00
MPG Goat-anti Mouse IgG F(c) G-AB12020 2 ml Inquire* 
G-AB1210010 mlInquire*
MPG Goat-anti Mouse IgM mu G-AB13020 2 ml Inquire*
G-AB1310010 mlInquire*
MPG Goat-anti Human IgG F(c) G-AB21020 2 ml Inquire*
G-AB2110010 mlInquire*
MPG Goat-anti Rabbit IgG F(c) G-AB31020 2 ml Inquire*
G-AB3110010 mlInquire*
MPG Goat-anti Rat IgG (H&L) G-AB41020 2 ml Inquire*
G-AB4110010 mlInquire*
MPG Donkey-anti Rabbit IgG (H&L) D–AB32020 2 ml Inquire*
G-AB3210010 mlInquire*
MPG Donkey-anti Goat IgG (H&L) D-AB51020 2 ml Inquire*
G-AB5110010 mlInquire*
MPG Donkey-anti Sheep IgG (H&L) D-AB61020 2 ml Inquire*
G-AB6110010 mlInquire*
MPG Rabbit-anti Human IgG (H&L) R-AB22020 2 ml Inquire*
G-AB2210010 mlInquire*
Components: Packed as 1% suspension in PBS with biocide and BSA.

*Please Inquire for more Information.

 

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Catalog #DescriptionUnitPrice
MPS0096 96-Well MPS (for 96-well microplates) 1 $300.00
MPS0301 3-in-1 MPS (holds 8 x 1.5 ml tubes or 1 x 15ml & 1 x 1.5 ml tube or 1 x 50 ml & 1 x 1.5 ml tube) 1$250.00
MPS0001 Mini Separator (holds 1 x 1.5 ml tube) 1 $40.00
  • UHMW polyethylene magnetic particle separator containing neodymium-iron-boron magnets

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