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Applications:
- Purification of nucleic acids
- Isolation of desired biomolecule
MPG™ Glyceryl has a non-ionic hydrophilic coating providing two adjacent hydroxyl groups for the covalent attachment of biomolecules containing primary and/or secondary amines. It is used for coupling antigens or antibodies for specific antibody or antigen capture when low non-specific binding is desirable. Biomolecules are covalently coupled to the MPG™ Glyceryl in a two-step procedure which begins with periodate oxidation of the hydroxyl groups on the particle surface to form aldehydes. The amine-containing biomolecule is then covalently linked to the aldehyde-modified MPG™ by reductive amination. Since the surface hydroxyl groups are non-ionic, non-specific binding to the particle surface is minimized.
Following the suggested protocol, coupling efficiencies of up to 75 µg of antibody per mg of MPG™ Glyceryl are achieved.
Related Sections
| Catalog # |
Description |
Unit |
Price |
| MGLY0502 |
MPG™ Glyceryl |
2 ml (60 mg) |
$125.00 |
| MGLY0510 |
MPG™ Glyceryl |
10 ml (300 mg) |
$400.00 |
     
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