How Magnetic Technology Works
Magnetic technology provides a rapid, reliable and reproducible means for separation and isolation of biomolecules. Magnetic Porous Glass (MPG™)1 products for magnetic separations are simple to use and cost effective. Use of MPG™ Products eliminates the need for centrifugation and column chromatography.
Magnetic separations offer the ability to process your samples in a single tube as illustrated in the diagram above. This single tube approach eliminates potential sample loss during tube transfers required in other separation methods. Processing your sample in a single tube also makes it simpler to run simultaneous samples. For high throughput applications, the process can be conducted in microplates. The magnetic properties of the particles enable concentration of target molecules as opposed to dilution of target molecules that occurs with column chromatography.
The gentler nature of a magnetic separation eliminates shear forces, which occur with centrifugation, preventing possible damage to RNA and DNA. This gentler separation technique also minimizes trapping of undesired products associated with other separation methods.
MPG™ is a spheroidal, totally porous borosilicate glass particle embedded with superparamagnetic iron oxide. MPG™ is made through a proprietary, patented process using Controlled Pore Glass (CPG)2. MPG™ has similar properties to CPG providing the same advantages as this glass matrix. The porosity of MPG™ provides a large surface area which results in a high capacity for ligand binding. The surface of MPG™ may be easily derivatized to produce various functional groups by using appropriate silanes. MPG™ is hydrophilic and can be dried or frozen making long-term storage of immobilized biomolecules a reality. Dried MPG™ can be easily resuspended in liquid without the problem of irreversible aggregation. MPG™ is heat-stable and amenable to temperature cycling reactions as in the Polymerase Chain Reaction. MPG™ is also inert to solvents.
1MPG™ is a registered trademark owned by EMD Millipore Corporation and licensed by PureBiotech, LLC.
2Patents 5601979, 5610274, and 5734020 owned by EMD Millipore Corporation and licensed by PureBiotech, LLC.
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