Frequently Asked TechTALK Questions

• How many mRNA isolations can be run with the MPG® Direct mRNA Purification Kits?

• Can the amount of mRNA isolated using the mRNA Isolation Kits be increased by reusing the particles?

• I want to use an immobilized capture probe to isolate a specific DNA species. Which product is best suited for this purpose?

• How much nucleic acid can be covalently coupled to MPG® LCA using the Solid Phase Oligo COUPLE-IT™ Kit?

• How do I optimize my PCR conditions when using the Taq-FORCE™ DNA Amplification System?

• I want to use the SOLIDscript™ Solid Phase cDNA Synthesis Kit to construct immobilized cDNA libraries. Which RNA isolation kit can I use that is compatible with this solid phase cDNA synthesis kit?

• How much mRNA can I expect to isolate from tissue or cells? What is the proportion of mRNA to total RNA?

• Why does isolated RNA degrade so easily and how can I prevent this?

• How can I efficiently produce a cDNA library without vector background?

• By what methodology does PureBiotech LLC's DNA-Pure™ PCR Clean Up Kit purify PCR products?

• I have been using PureBiotech LLC's Solidscript™ Solid Phase cDNA Synthesis Kit to study gene expression. I now want to isolate specific rare messenger RNAs using complementary probes. Can I still use this kit?

• I am synthesizing a 60' mer 5' amine-modified oligonucleotide to be used to covalently couple to magnetic particles using the Solid Phase Oligo Couple-IT™ Kit. Since only approximately 60% of the oligos will be full length, do I need to purify the oligo before coupling?

• There are many different ratios and pH of phenol:chloroform some with isoamyl alcohol. How do I choose the correct formulations?

• During DNA isolation I have a hard time recovering the aqueous phase without contamination from the interface of the phenol/chloroform extraction step. How can I avoid this contamination and still recover the entire DNA from the aqueous phase?

• I need to generate a cDNA library from only a few milligrams of an aortic tissue biopsy. Is this possible using your MPG® mRNA Purification Kits and can I scale your protocols down linearly?

• I want to purify protein using your Spin-Pure™ Columns. How do I decide which type to use?

• I have a peptide, which binds to a factor in serum. Can I conjugate this peptide to MPG® Long Chain Alkylamine to affinity purify the factor?