PureBiotech LLC's RNase Inhibitor is a recombinant 50 kD protein purified from an overexpressing strain of E. coli. This enzyme inhibits RNase by tightly binding (K_a~10¹⁴M) RNase A-type enzymes in a 1:1 molar ratio. Our RNase Inhibitor does not inhibit nucleic acid modifying enzymes commonly used in molecular biology applications, such as; AMV reverse transcriptase, MMLV reverse transcriptase, Taq DNA polymerase, phage RNA polymerases, RNase H, RNase T1, or S1 nuclease. Use of RNase Inhibitor increases yields in cDNA synthesis reactions, in vitro transcription, in vitro translation, and other reactions that utilize RNA substrates or produce RNA.

Storage Buffer: 20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, 50% glycerol.

Storage Conditions: Store at -20°C. Avoid frequent temperature changes.

Reaction Buffer: Active over pH range of 5.0 to 9.0; requires >= 1mM DTT.

Concentration: 20 U/µl

Unit Definition: One unit of RNase Inhibitor inhibits the activity of 5 ng of RNase A by 50% using cytidine 2',3'-cyclic monophosphate as a substrate.

Physical Purity: Greater than 90% by SDS-PAGE. Free of detectable nucleases.