Quality Control Analysis:
Activity of all Taq-FORCE™ DNA Polymerases are used in standard applications to measure yield and specificity. Enzymes are tested for the presence of endonuclease activity by incubation with lambda DNA; for nickases by incubation with supercoiled plasmid DNA; and for exonuclease by incubation with Hind III-digested lambda DNA. Reactions contain 1 µg DNA and 5 units of enzyme and are incubated for 8 hours at 72°C in the appropriate complete reaction buffer. The reactions are analyzed by gel electrophoresis to confirm no change in banding patterns.

Unit Definition:
For all Taq-FORCE™ DNA Polymerases one unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid-insoluble form in 30 min. at 72°C under the following assay conditions: 25mM TAPS (tris-[hydroxymethyl]methylamino-propanesulphonic acid, sodium salt), pH 9.3 at 25°C, 50mM KCl, 2mM MgCl₂, 1mM b-mercaptoethanol, 200µM each of dATP, dGTP, dTTP, 100µM dCTP (a mix of unlabeled and _-[³²P]-labeled) and 12.5µg activated salmon sperm DNA in a final volume of 50µl.

Storage Buffer and Conditions:
All Taq-FORCE™ DNA Polymerases are stored in 20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% glycerol, 0.1% Tween-20. Taq-FORCE™ Red storage buffer also contains an inert dye. These enzymes will remain stable at -20°C in a constant-temperature freezer.

Available Taq-FORCE™ Enzymes

• Taq-FORCE™ Amplification System

• Taq-FORCE™ Unmodified

• Taq-FORCE™ Red

• Taq-FORCE™ Long

• Taq-FORCE™ Hot