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Decontaminate PCR work areas
Ready-to-use
Cost effective

Non-carcinogenic
Heat resistant

DNA-OFF® is a non-alkaline cleansing solution that is highly active against DNA contamination. This solution contains a surfactant plus an agent that destroys DNA. DNA-OFF® is intended for use at PCR workstations, including countertops, apparatus and pipettors. Presence of contaminant DNA at a PCR work area can result in DNA amplification artifacts. DNA-OFF® has been demonstrated to effectively remove DNA contamination (

30 pg p53 DNA template) from a countertop as evidenced by the inability to generate a PCR product after cleaning (Figure 1).

The DNA-OFF® decontamination solution is stable and heat resistant. In addition, DNA-OFF® does not contain carcinogenic compounds.

DNA-OFF® is ready-to-use for eliminating DNA from all surfaces. The decontamination instructions are easy to follow. By simply applying DNA-OFF® and rinsing, DNA is completely removed leaving your work area free of contaminant DNA. It is available in a spray bottle and also an economical refill size.

Figure 1

Effectiveness of DNA-OFF® at removal of DNA contamination. Increasing amounts of p53 template DNA were dried onto the bottom of thin wall PCR tubes. DNA-OFF® (100 µl) was added to the tubes as indicated and 100 µl ddH₂O was added to the control tube. Each tube was vortexed for 20 seconds followed by two 100 µl washes with ddH₂O. PCR reaction mix containing 15 pg IL-1b DNA template and both IL-1b and p53 were added to each tube and run for 35 cycles. PCR products were resolved on a 1.2% agarose gel (1X TAE) and visualized by EtBr staining.

Catalog #	Description	Unit	Price
DO251	DNA-OFF® Spray Bottle	250 ml	$28.55
DO252	DNA-OFF® Spray Bottle and Refill	250 ml & 500 ml Bundle	$56.60
DO500	DNA-OFF® Refill Bottle	500 ml	$37.34

DNA-OFF® is a registered trademark of PureBiotech LLC.