Applications:

• Isolation and/or purification of biomolecules
• Isolation and purification of RNA binding proteins
• Enzyme assays

The linkage, to the particle surface, is accomplished in two steps. First, the carbohydrate containing vicinal hydroxyl moieties on the ligand are oxidized to form aldehyde groups. The newly formed aldehyde groups are then covalently bound to the surface of the MPG^™ Hydrazide particles by forming stable hydrazone bonds.

The major advantage of a hydrazide linkage is the site-specific orientation of the coupled ligand. For example, antibodies are linked through the carbohydrate side chain on the Fc portion of the molecule leaving both antigen binding sites accessible; and RNA is specifically immobilized to its 3' end by oxidation of the vicinal 2' and 3' hydroxyl groups on the ribose. Another advantage of hydrazide chemistry is the elimination of a sodium borohydride step, which may cause reduction of the carbonyl groups present on the ligand, thereby lowering its biological activity. In addition, glycolipids, proteoglycans and molecules containing aliphatic aldehydes can be coupled to MPG^™ Hydrazide. Typical hydrazide loading range from 75 to 125 nmole/mg MPG^™.

Related Sections

• Magnetic Technology
• Magnetic Separators